Analysis of Certain Errors in Squid Axon Voltage Clamp Measurements
نویسندگان
چکیده
منابع مشابه
Nonuniform response in the squid axon membrana under voltage-clamp.
TASAKT, I. AND C. S. SPYROPOULOS. Nonunijorm response in the squid axon membrane under ‘voltage-clamp.’ Am. J. Physiol. 193(2): 309-317. 1958.-By cooling a portion of the squid giant axon at a time, it was shown that the repetitive membrane currents observed under the so-called voltageclamp conditions derived from the main portion of the ‘clamped’ axon membrane and not from the lateral unclampe...
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,4BSTRACT TASAKI, I. AND C. S. SPYROPOULOS. Membrane conductance and currentvoltage relation in the squid axon under ‘voltage-clamp.’ &L-The conductance of the squid axon membrane under ‘voltage-clamp’ was measured by superposing a sinusoidal wave upon rectangular clamping voltage pulses. It was possible to determine the time course of the emf of the membrane under ‘voltage-clamp’ on a single p...
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TASAKI, I. AND A. F. BAK. Discrete threshold and repetitive responses in the squid axon under ‘voltage-claw@ By using the so-called voltage-clamp technique, records were obtained indicating the presence of undulatory or oscillatory membrane current under fixed potential difference between the internal and external electrodes. This oscillatory membrane current was observed only when rectangular ...
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A new voltage-clamp apparatus for the squid axon has been implemented to enable recording of currents through small areas of axon membrane. The performance of this clamp was tested by recording total sodium currents from perfused axons (I total) and sodium currents from small membrane patches (I patch), which were recorded from inside the axon with an L-shaped pipette. The I patch records, alth...
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Isolated and cleaned giant axons of Loligo pealii were internally perfused with solutions containing cesium sulfate and potassium fluoride. Membrane currents obtained as a function of clamped membrane potentials indicated a severe depression of the delayed outward current component normally attributed to potassium ion movement. Steady-state currents showed a negative slope in the potential rang...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 1960
ISSN: 0006-3495
DOI: 10.1016/s0006-3495(60)86882-8